Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
70 |
8.1% |
| Arg[R] |
39 |
4.5% |
| Asn[N] |
41 |
4.7% |
| Asp[D] |
49 |
5.6% |
| Cys[C] |
13 |
1.5% |
| Gln[Q] |
34 |
3.9% |
| Glu[E] |
58 |
6.7% |
| Gly[G] |
48 |
5.5% |
| His[H] |
19 |
2.2% |
| Ile[I] |
58 |
6.7% |
| Leu[L] |
67 |
7.7% |
| Lys[K] |
51 |
5.9% |
| Met[M] |
26 |
3.0% |
| Phe[F] |
31 |
3.6% |
| Pro[P] |
41 |
4.7% |
| Ser[S] |
66 |
7.6% |
| Thr[T] |
61 |
7.0% |
| Trp[W] |
12 |
1.4% |
| Tyr[Y] |
37 |
4.3% |
| Val[V] |
48 |
5.5% |
| Total Length |
869 |
|
| Instability index (II) |
46.56 |
| This classifies the protein as unstable |
|
| Carbon |
4330 |
| Hydrogen |
6760 |
| Nitrogen |
1162 |
| Oxygen |
1323 |
| Sulfur |
39 |
| Total number of atoms |
13614 |
| Formula = C4330H6760N1162O1323S39 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
121880 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
121130 M-1 cm-1 |
| assuming all Cys residues are reduced |
121130 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
121880 M-1 cm-1 |
|
|
| Codon Bias |
0.307 |
| Codon Adaptation Index |
0.290 |
| Frequency of Optimal Codons |
0.590 |
| Hydropathicity of Protein |
-0.345 |
| Aromaticity Score |
0.092 |
|
