YIL058W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 6 6.4%
Arg[R] 4 4.3%
Asn[N] 5 5.3%
Asp[D] 1 1.1%
Cys[C] 8 8.5%
Gln[Q] 2 2.1%
Glu[E] 3 3.2%
Gly[G] 10 10.6%
His[H] 4 4.3%
Ile[I] 8 8.5%
Leu[L] 8 8.5%
Lys[K] 2 2.1%
Met[M] 4 4.3%
Phe[F] 4 4.3%
Pro[P] 4 4.3%
Ser[S] 4 4.3%
Thr[T] 2 2.1%
Trp[W] 0 0.0%
Tyr[Y] 6 6.4%
Val[V] 9 9.6%
Total Length 94

Instability index
Instability index (II) 31.90
This classifies the protein as stable

Atomic composition
Carbon 462
Hydrogen 719
Nitrogen 123
Oxygen 122
Sulfur 12
Total number of atoms 1438
Formula = C462H719N123O122S12

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 9440 M-1 cm-1
assuming NO Cys residues appear as half cystines 8940 M-1 cm-1
assuming all Cys residues are reduced 8940 M-1 cm-1
assuming all pairs of Cys residues form cystines 9440 M-1 cm-1

Aliphatic index
Aliphatic index 100.53

Coding Region Translation Calculations
Codon Bias 0.175
Codon Adaptation Index 0.145
Frequency of Optimal Codons 0.511
Hydropathicity of Protein 0.573
Aromaticity Score 0.106