Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
10 |
4.0% |
| Arg[R] |
9 |
3.6% |
| Asn[N] |
12 |
4.7% |
| Asp[D] |
4 |
1.6% |
| Cys[C] |
5 |
2.0% |
| Gln[Q] |
4 |
1.6% |
| Glu[E] |
10 |
4.0% |
| Gly[G] |
5 |
2.0% |
| His[H] |
8 |
3.2% |
| Ile[I] |
25 |
9.9% |
| Leu[L] |
34 |
13.4% |
| Lys[K] |
15 |
5.9% |
| Met[M] |
3 |
1.2% |
| Phe[F] |
19 |
7.5% |
| Pro[P] |
11 |
4.3% |
| Ser[S] |
20 |
7.9% |
| Thr[T] |
14 |
5.5% |
| Trp[W] |
5 |
2.0% |
| Tyr[Y] |
27 |
10.7% |
| Val[V] |
13 |
5.1% |
| Total Length |
253 |
|
| Instability index (II) |
41.06 |
| This classifies the protein as unstable |
|
| Carbon |
1455 |
| Hydrogen |
2162 |
| Nitrogen |
332 |
| Oxygen |
359 |
| Sulfur |
8 |
| Total number of atoms |
4316 |
| Formula = C1455H2162N332O359S8 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
67980 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
67730 M-1 cm-1 |
| assuming all Cys residues are reduced |
67730 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
67980 M-1 cm-1 |
|
|
| Codon Bias |
-0.053 |
| Codon Adaptation Index |
0.104 |
| Frequency of Optimal Codons |
0.388 |
| Hydropathicity of Protein |
0.281 |
| Aromaticity Score |
0.202 |
|
