Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
75 |
5.7% |
| Arg[R] |
61 |
4.6% |
| Asn[N] |
75 |
5.7% |
| Asp[D] |
74 |
5.6% |
| Cys[C] |
18 |
1.4% |
| Gln[Q] |
41 |
3.1% |
| Glu[E] |
71 |
5.4% |
| Gly[G] |
60 |
4.5% |
| His[H] |
29 |
2.2% |
| Ile[I] |
109 |
8.3% |
| Leu[L] |
140 |
10.6% |
| Lys[K] |
64 |
4.9% |
| Met[M] |
38 |
2.9% |
| Phe[F] |
88 |
6.7% |
| Pro[P] |
64 |
4.9% |
| Ser[S] |
91 |
6.9% |
| Thr[T] |
59 |
4.5% |
| Trp[W] |
18 |
1.4% |
| Tyr[Y] |
59 |
4.5% |
| Val[V] |
85 |
6.4% |
| Total Length |
1319 |
|
| Instability index (II) |
38.43 |
| This classifies the protein as stable |
|
| Carbon |
6938 |
| Hydrogen |
10660 |
| Nitrogen |
1758 |
| Oxygen |
1935 |
| Sulfur |
56 |
| Total number of atoms |
21347 |
| Formula = C6938H10660N1758O1935S56 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
188035 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
186910 M-1 cm-1 |
| assuming all Cys residues are reduced |
186910 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
188035 M-1 cm-1 |
|
|
| Codon Bias |
0.122 |
| Codon Adaptation Index |
0.169 |
| Frequency of Optimal Codons |
0.482 |
| Hydropathicity of Protein |
0.011 |
| Aromaticity Score |
0.125 |
|
