Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
55 |
4.8% |
| Arg[R] |
42 |
3.7% |
| Asn[N] |
67 |
5.8% |
| Asp[D] |
91 |
7.9% |
| Cys[C] |
11 |
1.0% |
| Gln[Q] |
47 |
4.1% |
| Glu[E] |
103 |
9.0% |
| Gly[G] |
20 |
1.7% |
| His[H] |
24 |
2.1% |
| Ile[I] |
91 |
7.9% |
| Leu[L] |
131 |
11.4% |
| Lys[K] |
98 |
8.5% |
| Met[M] |
16 |
1.4% |
| Phe[F] |
57 |
5.0% |
| Pro[P] |
34 |
3.0% |
| Ser[S] |
95 |
8.3% |
| Thr[T] |
67 |
5.8% |
| Trp[W] |
10 |
0.9% |
| Tyr[Y] |
33 |
2.9% |
| Val[V] |
58 |
5.0% |
| Total Length |
1150 |
|
| Instability index (II) |
41.39 |
| This classifies the protein as unstable |
|
| Carbon |
5949 |
| Hydrogen |
9394 |
| Nitrogen |
1546 |
| Oxygen |
1848 |
| Sulfur |
27 |
| Total number of atoms |
18764 |
| Formula = C5949H9394N1546O1848S27 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
104795 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
104170 M-1 cm-1 |
| assuming all Cys residues are reduced |
104170 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
104795 M-1 cm-1 |
|
|
| Codon Bias |
0.078 |
| Codon Adaptation Index |
0.169 |
| Frequency of Optimal Codons |
0.469 |
| Hydropathicity of Protein |
-0.431 |
| Aromaticity Score |
0.087 |
|
