URM1/YIL008W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 1 1.0%
Arg[R] 2 2.0%
Asn[N] 5 5.1%
Asp[D] 13 13.1%
Cys[C] 0 0.0%
Gln[Q] 1 1.0%
Glu[E] 7 7.1%
Gly[G] 9 9.1%
His[H] 3 3.0%
Ile[I] 14 14.1%
Leu[L] 7 7.1%
Lys[K] 6 6.1%
Met[M] 3 3.0%
Phe[F] 4 4.0%
Pro[P] 3 3.0%
Ser[S] 5 5.1%
Thr[T] 6 6.1%
Trp[W] 1 1.0%
Tyr[Y] 1 1.0%
Val[V] 8 8.1%
Total Length 99

Instability index
Instability index (II) 31.34
This classifies the protein as stable

Atomic composition
Carbon 490
Hydrogen 776
Nitrogen 124
Oxygen 158
Sulfur 3
Total number of atoms 1551
Formula = C490H776N124O158S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 6990 M-1 cm-1

Aliphatic index
Aliphatic index 107.17

Coding Region Translation Calculations
Codon Bias 0.139
Codon Adaptation Index 0.205
Frequency of Optimal Codons 0.526
Hydropathicity of Protein -0.100
Aromaticity Score 0.061