YHR217C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 0 0.0%
Arg[R] 2 1.3%
Asn[N] 3 2.0%
Asp[D] 1 0.7%
Cys[C] 3 2.0%
Gln[Q] 2 1.3%
Glu[E] 0 0.0%
Gly[G] 0 0.0%
His[H] 24 15.7%
Ile[I] 9 5.9%
Leu[L] 17 11.1%
Lys[K] 1 0.7%
Met[M] 2 1.3%
Phe[F] 0 0.0%
Pro[P] 35 22.9%
Ser[S] 13 8.5%
Thr[T] 30 19.6%
Trp[W] 0 0.0%
Tyr[Y] 7 4.6%
Val[V] 4 2.6%
Total Length 153

Instability index
Instability index (II) 43.11
This classifies the protein as unstable

Atomic composition
Carbon 780
Hydrogen 1183
Nitrogen 213
Oxygen 211
Sulfur 5
Total number of atoms 2392
Formula = C780H1183N213O211S5

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 10555 M-1 cm-1
assuming NO Cys residues appear as half cystines 10430 M-1 cm-1
assuming all Cys residues are reduced 10430 M-1 cm-1
assuming all pairs of Cys residues form cystines 10555 M-1 cm-1

Aliphatic index
Aliphatic index 73.86

Coding Region Translation Calculations
Codon Bias 0.321
Codon Adaptation Index 0.174
Frequency of Optimal Codons 0.589
Hydropathicity of Protein -0.484
Aromaticity Score 0.046