Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
47 |
4.4% |
| Arg[R] |
14 |
1.3% |
| Asn[N] |
32 |
3.0% |
| Asp[D] |
27 |
2.5% |
| Cys[C] |
17 |
1.6% |
| Gln[Q] |
31 |
2.9% |
| Glu[E] |
60 |
5.6% |
| Gly[G] |
71 |
6.6% |
| His[H] |
6 |
0.6% |
| Ile[I] |
72 |
6.7% |
| Leu[L] |
39 |
3.6% |
| Lys[K] |
18 |
1.7% |
| Met[M] |
15 |
1.4% |
| Phe[F] |
26 |
2.4% |
| Pro[P] |
57 |
5.3% |
| Ser[S] |
175 |
16.3% |
| Thr[T] |
257 |
23.9% |
| Trp[W] |
15 |
1.4% |
| Tyr[Y] |
25 |
2.3% |
| Val[V] |
71 |
6.6% |
| Total Length |
1075 |
|
| Instability index (II) |
36.77 |
| This classifies the protein as stable |
|
| Carbon |
4811 |
| Hydrogen |
7633 |
| Nitrogen |
1225 |
| Oxygen |
1770 |
| Sulfur |
32 |
| Total number of atoms |
15471 |
| Formula = C4811H7633N1225O1770S32 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
120750 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
119750 M-1 cm-1 |
| assuming all Cys residues are reduced |
119750 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
120750 M-1 cm-1 |
|
|
| Codon Bias |
0.341 |
| Codon Adaptation Index |
0.233 |
| Frequency of Optimal Codons |
0.621 |
| Hydropathicity of Protein |
-0.153 |
| Aromaticity Score |
0.061 |
|
