Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
5.2% |
| Arg[R] |
24 |
2.9% |
| Asn[N] |
79 |
9.6% |
| Asp[D] |
56 |
6.8% |
| Cys[C] |
9 |
1.1% |
| Gln[Q] |
38 |
4.6% |
| Glu[E] |
41 |
5.0% |
| Gly[G] |
52 |
6.3% |
| His[H] |
31 |
3.8% |
| Ile[I] |
39 |
4.7% |
| Leu[L] |
60 |
7.3% |
| Lys[K] |
49 |
5.9% |
| Met[M] |
20 |
2.4% |
| Phe[F] |
43 |
5.2% |
| Pro[P] |
39 |
4.7% |
| Ser[S] |
84 |
10.2% |
| Thr[T] |
54 |
6.6% |
| Trp[W] |
8 |
1.0% |
| Tyr[Y] |
20 |
2.4% |
| Val[V] |
35 |
4.2% |
| Total Length |
824 |
|
| Instability index (II) |
33.89 |
| This classifies the protein as stable |
|
| Carbon |
4006 |
| Hydrogen |
6158 |
| Nitrogen |
1132 |
| Oxygen |
1294 |
| Sulfur |
29 |
| Total number of atoms |
12619 |
| Formula = C4006H6158N1132O1294S29 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
74300 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
73800 M-1 cm-1 |
| assuming all Cys residues are reduced |
73800 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
74300 M-1 cm-1 |
|
|
| Codon Bias |
0.105 |
| Codon Adaptation Index |
0.170 |
| Frequency of Optimal Codons |
0.475 |
| Hydropathicity of Protein |
-0.679 |
| Aromaticity Score |
0.086 |
|
