RIX1/YHR197W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 28 3.7%
Arg[R] 24 3.1%
Asn[N] 43 5.6%
Asp[D] 32 4.2%
Cys[C] 9 1.2%
Gln[Q] 26 3.4%
Glu[E] 73 9.6%
Gly[G] 28 3.7%
His[H] 18 2.4%
Ile[I] 66 8.7%
Leu[L] 99 13.0%
Lys[K] 59 7.7%
Met[M] 11 1.4%
Phe[F] 37 4.8%
Pro[P] 34 4.5%
Ser[S] 66 8.7%
Thr[T] 42 5.5%
Trp[W] 6 0.8%
Tyr[Y] 15 2.0%
Val[V] 47 6.2%
Total Length 763

Instability index
Instability index (II) 44.85
This classifies the protein as unstable

Atomic composition
Carbon 3918
Hydrogen 6257
Nitrogen 1005
Oxygen 1166
Sulfur 20
Total number of atoms 12366
Formula = C3918H6257N1005O1166S20

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 55850 M-1 cm-1
assuming NO Cys residues appear as half cystines 55350 M-1 cm-1
assuming all Cys residues are reduced 55350 M-1 cm-1
assuming all pairs of Cys residues form cystines 55850 M-1 cm-1

Aliphatic index
Aliphatic index 105.87

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000