EGD2/YHR193C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 24 13.8%
Arg[R] 4 2.3%
Asn[N] 14 8.0%
Asp[D] 12 6.9%
Cys[C] 0 0.0%
Gln[Q] 12 6.9%
Glu[E] 15 8.6%
Gly[G] 11 6.3%
His[H] 1 0.6%
Ile[I] 12 6.9%
Leu[L] 10 5.7%
Lys[K] 16 9.2%
Met[M] 4 2.3%
Phe[F] 4 2.3%
Pro[P] 5 2.9%
Ser[S] 9 5.2%
Thr[T] 5 2.9%
Trp[W] 0 0.0%
Tyr[Y] 2 1.1%
Val[V] 14 8.0%
Total Length 174

Instability index
Instability index (II) 35.82
This classifies the protein as stable

Atomic composition
Carbon 807
Hydrogen 1320
Nitrogen 230
Oxygen 271
Sulfur 4
Total number of atoms 2632
Formula = C807H1320N230O271S4

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 2980 M-1 cm-1

Aliphatic index
Aliphatic index 86.44

Coding Region Translation Calculations
Codon Bias 0.617
Codon Adaptation Index 0.578
Frequency of Optimal Codons 0.776
Hydropathicity of Protein -0.471
Aromaticity Score 0.034