Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
5.7% |
| Arg[R] |
25 |
4.1% |
| Asn[N] |
26 |
4.3% |
| Asp[D] |
41 |
6.7% |
| Cys[C] |
8 |
1.3% |
| Gln[Q] |
21 |
3.4% |
| Glu[E] |
31 |
5.1% |
| Gly[G] |
29 |
4.8% |
| His[H] |
13 |
2.1% |
| Ile[I] |
33 |
5.4% |
| Leu[L] |
77 |
12.6% |
| Lys[K] |
27 |
4.4% |
| Met[M] |
17 |
2.8% |
| Phe[F] |
35 |
5.7% |
| Pro[P] |
37 |
6.1% |
| Ser[S] |
55 |
9.0% |
| Thr[T] |
47 |
7.7% |
| Trp[W] |
8 |
1.3% |
| Tyr[Y] |
21 |
3.4% |
| Val[V] |
24 |
3.9% |
| Total Length |
610 |
|
| Instability index (II) |
40.46 |
| This classifies the protein as unstable |
|
| Carbon |
3100 |
| Hydrogen |
4791 |
| Nitrogen |
793 |
| Oxygen |
925 |
| Sulfur |
25 |
| Total number of atoms |
9634 |
| Formula = C3100H4791N793O925S25 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
75790 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
75290 M-1 cm-1 |
| assuming all Cys residues are reduced |
75290 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
75790 M-1 cm-1 |
|
|
| Codon Bias |
0.114 |
| Codon Adaptation Index |
0.140 |
| Frequency of Optimal Codons |
0.462 |
| Hydropathicity of Protein |
-0.169 |
| Aromaticity Score |
0.105 |
|
