GND1/YHR183W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 47 9.6%
Arg[R] 21 4.3%
Asn[N] 21 4.3%
Asp[D] 31 6.3%
Cys[C] 7 1.4%
Gln[Q] 14 2.9%
Glu[E] 27 5.5%
Gly[G] 47 9.6%
His[H] 9 1.8%
Ile[I] 36 7.4%
Leu[L] 42 8.6%
Lys[K] 33 6.7%
Met[M] 11 2.2%
Phe[F] 22 4.5%
Pro[P] 21 4.3%
Ser[S] 30 6.1%
Thr[T] 19 3.9%
Trp[W] 8 1.6%
Tyr[Y] 15 3.1%
Val[V] 28 5.7%
Total Length 489

Instability index
Instability index (II) 33.63
This classifies the protein as stable

Atomic composition
Carbon 2402
Hydrogen 3758
Nitrogen 646
Oxygen 705
Sulfur 18
Total number of atoms 7529
Formula = C2402H3758N646O705S18

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 66725 M-1 cm-1
assuming NO Cys residues appear as half cystines 66350 M-1 cm-1
assuming all Cys residues are reduced 66350 M-1 cm-1
assuming all pairs of Cys residues form cystines 66725 M-1 cm-1

Aliphatic index
Aliphatic index 88.43

Coding Region Translation Calculations
Codon Bias 0.676
Codon Adaptation Index 0.623
Frequency of Optimal Codons 0.804
Hydropathicity of Protein -0.143
Aromaticity Score 0.092