Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
11 |
3.9% |
| Arg[R] |
11 |
3.9% |
| Asn[N] |
32 |
11.3% |
| Asp[D] |
18 |
6.4% |
| Cys[C] |
3 |
1.1% |
| Gln[Q] |
10 |
3.5% |
| Glu[E] |
26 |
9.2% |
| Gly[G] |
15 |
5.3% |
| His[H] |
7 |
2.5% |
| Ile[I] |
18 |
6.4% |
| Leu[L] |
22 |
7.8% |
| Lys[K] |
18 |
6.4% |
| Met[M] |
4 |
1.4% |
| Phe[F] |
18 |
6.4% |
| Pro[P] |
11 |
3.9% |
| Ser[S] |
29 |
10.2% |
| Thr[T] |
14 |
4.9% |
| Trp[W] |
2 |
0.7% |
| Tyr[Y] |
4 |
1.4% |
| Val[V] |
10 |
3.5% |
| Total Length |
283 |
|
| Instability index (II) |
46.34 |
| This classifies the protein as unstable |
|
| Carbon |
1396 |
| Hydrogen |
2162 |
| Nitrogen |
392 |
| Oxygen |
461 |
| Sulfur |
7 |
| Total number of atoms |
4418 |
| Formula = C1396H2162N392O461S7 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
17085 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
16960 M-1 cm-1 |
| assuming all Cys residues are reduced |
16960 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
17085 M-1 cm-1 |
|
|
| Codon Bias |
-0.021 |
| Codon Adaptation Index |
0.131 |
| Frequency of Optimal Codons |
0.408 |
| Hydropathicity of Protein |
-0.759 |
| Aromaticity Score |
0.085 |
|
