TRR2/YHR106W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 35 10.2%
Arg[R] 16 4.7%
Asn[N] 14 4.1%
Asp[D] 14 4.1%
Cys[C] 4 1.2%
Gln[Q] 9 2.6%
Glu[E] 24 7.0%
Gly[G] 28 8.2%
His[H] 7 2.0%
Ile[I] 26 7.6%
Leu[L] 23 6.7%
Lys[K] 20 5.8%
Met[M] 10 2.9%
Phe[F] 14 4.1%
Pro[P] 14 4.1%
Ser[S] 26 7.6%
Thr[T] 24 7.0%
Trp[W] 2 0.6%
Tyr[Y] 9 2.6%
Val[V] 23 6.7%
Total Length 342

Instability index
Instability index (II) 41.02
This classifies the protein as unstable

Atomic composition
Carbon 1640
Hydrogen 2615
Nitrogen 449
Oxygen 501
Sulfur 14
Total number of atoms 5219
Formula = C1640H2615N449O501S14

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 24660 M-1 cm-1
assuming NO Cys residues appear as half cystines 24410 M-1 cm-1
assuming all Cys residues are reduced 24410 M-1 cm-1
assuming all pairs of Cys residues form cystines 24660 M-1 cm-1

Aliphatic index
Aliphatic index 85.61

Coding Region Translation Calculations
Codon Bias 0.118
Codon Adaptation Index 0.138
Frequency of Optimal Codons 0.473
Hydropathicity of Protein -0.112
Aromaticity Score 0.073