Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
202 |
5.4% |
| Arg[R] |
167 |
4.5% |
| Asn[N] |
243 |
6.5% |
| Asp[D] |
208 |
5.6% |
| Cys[C] |
37 |
1.0% |
| Gln[Q] |
156 |
4.2% |
| Glu[E] |
244 |
6.5% |
| Gly[G] |
90 |
2.4% |
| His[H] |
83 |
2.2% |
| Ile[I] |
289 |
7.7% |
| Leu[L] |
490 |
13.1% |
| Lys[K] |
243 |
6.5% |
| Met[M] |
90 |
2.4% |
| Phe[F] |
212 |
5.7% |
| Pro[P] |
163 |
4.4% |
| Ser[S] |
264 |
7.1% |
| Thr[T] |
201 |
5.4% |
| Trp[W] |
41 |
1.1% |
| Tyr[Y] |
130 |
3.5% |
| Val[V] |
191 |
5.1% |
| Total Length |
3744 |
|
| Instability index (II) |
43.72 |
| This classifies the protein as unstable |
|
| Carbon |
19678 |
| Hydrogen |
30876 |
| Nitrogen |
5094 |
| Oxygen |
5643 |
| Sulfur |
127 |
| Total number of atoms |
61418 |
| Formula = C19678H30876N5094O5643S127 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
421450 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
419200 M-1 cm-1 |
| assuming all Cys residues are reduced |
419200 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
421450 M-1 cm-1 |
|
|
| Codon Bias |
0.046 |
| Codon Adaptation Index |
0.139 |
| Frequency of Optimal Codons |
0.436 |
| Hydropathicity of Protein |
-0.164 |
| Aromaticity Score |
0.102 |
|
