Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
33 |
4.8% |
| Arg[R] |
27 |
3.9% |
| Asn[N] |
54 |
7.8% |
| Asp[D] |
42 |
6.1% |
| Cys[C] |
7 |
1.0% |
| Gln[Q] |
38 |
5.5% |
| Glu[E] |
41 |
6.0% |
| Gly[G] |
28 |
4.1% |
| His[H] |
9 |
1.3% |
| Ile[I] |
19 |
2.8% |
| Leu[L] |
48 |
7.0% |
| Lys[K] |
44 |
6.4% |
| Met[M] |
19 |
2.8% |
| Phe[F] |
33 |
4.8% |
| Pro[P] |
65 |
9.4% |
| Ser[S] |
68 |
9.9% |
| Thr[T] |
42 |
6.1% |
| Trp[W] |
5 |
0.7% |
| Tyr[Y] |
32 |
4.7% |
| Val[V] |
34 |
4.9% |
| Total Length |
688 |
|
| Instability index (II) |
58.69 |
| This classifies the protein as unstable |
|
| Carbon |
3439 |
| Hydrogen |
5264 |
| Nitrogen |
928 |
| Oxygen |
1089 |
| Sulfur |
26 |
| Total number of atoms |
10746 |
| Formula = C3439H5264N928O1089S26 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
75555 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
75180 M-1 cm-1 |
| assuming all Cys residues are reduced |
75180 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
75555 M-1 cm-1 |
|
|
| Codon Bias |
0.065 |
| Codon Adaptation Index |
0.142 |
| Frequency of Optimal Codons |
0.441 |
| Hydropathicity of Protein |
-0.819 |
| Aromaticity Score |
0.102 |
|
