Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
40 |
3.6% |
| Arg[R] |
60 |
5.4% |
| Asn[N] |
69 |
6.2% |
| Asp[D] |
72 |
6.5% |
| Cys[C] |
14 |
1.3% |
| Gln[Q] |
33 |
3.0% |
| Glu[E] |
72 |
6.5% |
| Gly[G] |
54 |
4.8% |
| His[H] |
25 |
2.2% |
| Ile[I] |
80 |
7.2% |
| Leu[L] |
124 |
11.1% |
| Lys[K] |
72 |
6.5% |
| Met[M] |
26 |
2.3% |
| Phe[F] |
52 |
4.7% |
| Pro[P] |
54 |
4.8% |
| Ser[S] |
114 |
10.2% |
| Thr[T] |
49 |
4.4% |
| Trp[W] |
8 |
0.7% |
| Tyr[Y] |
39 |
3.5% |
| Val[V] |
58 |
5.2% |
| Total Length |
1115 |
|
| Instability index (II) |
47.15 |
| This classifies the protein as unstable |
|
| Carbon |
5660 |
| Hydrogen |
8925 |
| Nitrogen |
1527 |
| Oxygen |
1708 |
| Sulfur |
40 |
| Total number of atoms |
17860 |
| Formula = C5660H8925N1527O1708S40 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
102985 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
102110 M-1 cm-1 |
| assuming all Cys residues are reduced |
102110 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
102985 M-1 cm-1 |
|
|
| Codon Bias |
-0.009 |
| Codon Adaptation Index |
0.116 |
| Frequency of Optimal Codons |
0.399 |
| Hydropathicity of Protein |
-0.364 |
| Aromaticity Score |
0.089 |
|
