Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
25 |
6.2% |
| Arg[R] |
15 |
3.8% |
| Asn[N] |
22 |
5.5% |
| Asp[D] |
28 |
7.0% |
| Cys[C] |
4 |
1.0% |
| Gln[Q] |
15 |
3.8% |
| Glu[E] |
19 |
4.8% |
| Gly[G] |
22 |
5.5% |
| His[H] |
10 |
2.5% |
| Ile[I] |
26 |
6.5% |
| Leu[L] |
41 |
10.2% |
| Lys[K] |
30 |
7.5% |
| Met[M] |
4 |
1.0% |
| Phe[F] |
26 |
6.5% |
| Pro[P] |
14 |
3.5% |
| Ser[S] |
38 |
9.5% |
| Thr[T] |
26 |
6.5% |
| Trp[W] |
7 |
1.8% |
| Tyr[Y] |
7 |
1.8% |
| Val[V] |
21 |
5.2% |
| Total Length |
400 |
|
| Instability index (II) |
31.67 |
| This classifies the protein as stable |
|
| Carbon |
2020 |
| Hydrogen |
3147 |
| Nitrogen |
539 |
| Oxygen |
603 |
| Sulfur |
8 |
| Total number of atoms |
6317 |
| Formula = C2020H3147N539O603S8 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
49180 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
48930 M-1 cm-1 |
| assuming all Cys residues are reduced |
48930 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
49180 M-1 cm-1 |
|
|
| Codon Bias |
-0.007 |
| Codon Adaptation Index |
0.126 |
| Frequency of Optimal Codons |
0.404 |
| Hydropathicity of Protein |
-0.273 |
| Aromaticity Score |
0.100 |
|
