MED6/YHR058C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 13 4.4%
Arg[R] 12 4.1%
Asn[N] 26 8.8%
Asp[D] 13 4.4%
Cys[C] 0 0.0%
Gln[Q] 16 5.4%
Glu[E] 16 5.4%
Gly[G] 23 7.8%
His[H] 2 0.7%
Ile[I] 17 5.8%
Leu[L] 20 6.8%
Lys[K] 12 4.1%
Met[M] 14 4.7%
Phe[F] 10 3.4%
Pro[P] 16 5.4%
Ser[S] 22 7.5%
Thr[T] 30 10.2%
Trp[W] 3 1.0%
Tyr[Y] 12 4.1%
Val[V] 18 6.1%
Total Length 295

Instability index
Instability index (II) 46.93
This classifies the protein as unstable

Atomic composition
Carbon 1436
Hydrogen 2254
Nitrogen 392
Oxygen 460
Sulfur 14
Total number of atoms 4556
Formula = C1436H2254N392O460S14

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 34380 M-1 cm-1

Aliphatic index
Aliphatic index 71.02

Coding Region Translation Calculations
Codon Bias 0.119
Codon Adaptation Index 0.132
Frequency of Optimal Codons 0.482
Hydropathicity of Protein -0.479
Aromaticity Score 0.085