CUP1-1/YHR053C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 0 0.0%
Arg[R] 0 0.0%
Asn[N] 6 9.8%
Asp[D] 2 3.3%
Cys[C] 12 19.7%
Gln[Q] 5 8.2%
Glu[E] 6 9.8%
Gly[G] 5 8.2%
His[H] 1 1.6%
Ile[I] 1 1.6%
Leu[L] 1 1.6%
Lys[K] 7 11.5%
Met[M] 1 1.6%
Phe[F] 2 3.3%
Pro[P] 2 3.3%
Ser[S] 8 13.1%
Thr[T] 2 3.3%
Trp[W] 0 0.0%
Tyr[Y] 0 0.0%
Val[V] 0 0.0%
Total Length 61

Instability index
Instability index (II) 46.75
This classifies the protein as unstable

Atomic composition
Carbon 258
Hydrogen 413
Nitrogen 81
Oxygen 99
Sulfur 13
Total number of atoms 864
Formula = C258H413N81O99S13

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 750 M-1 cm-1
assuming NO Cys residues appear as half cystines 0 M-1 cm-1
assuming all Cys residues are reduced 0 M-1 cm-1
assuming all pairs of Cys residues form cystines 750 M-1 cm-1

Aliphatic index
Aliphatic index 12.79

Coding Region Translation Calculations
Codon Bias 0.128
Codon Adaptation Index 0.226
Frequency of Optimal Codons 0.517
Hydropathicity of Protein -1.052
Aromaticity Score 0.033