Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
41 |
5.7% |
| Arg[R] |
47 |
6.5% |
| Asn[N] |
33 |
4.6% |
| Asp[D] |
41 |
5.7% |
| Cys[C] |
4 |
0.6% |
| Gln[Q] |
30 |
4.1% |
| Glu[E] |
40 |
5.5% |
| Gly[G] |
35 |
4.8% |
| His[H] |
10 |
1.4% |
| Ile[I] |
48 |
6.6% |
| Leu[L] |
71 |
9.8% |
| Lys[K] |
66 |
9.1% |
| Met[M] |
19 |
2.6% |
| Phe[F] |
25 |
3.5% |
| Pro[P] |
27 |
3.7% |
| Ser[S] |
68 |
9.4% |
| Thr[T] |
43 |
5.9% |
| Trp[W] |
6 |
0.8% |
| Tyr[Y] |
17 |
2.4% |
| Val[V] |
52 |
7.2% |
| Total Length |
723 |
|
| Instability index (II) |
39.71 |
| This classifies the protein as stable |
|
| Carbon |
3613 |
| Hydrogen |
5897 |
| Nitrogen |
1019 |
| Oxygen |
1077 |
| Sulfur |
23 |
| Total number of atoms |
11629 |
| Formula = C3613H5897N1019O1077S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
58580 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
58330 M-1 cm-1 |
| assuming all Cys residues are reduced |
58330 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
58580 M-1 cm-1 |
|
|
| Codon Bias |
0.097 |
| Codon Adaptation Index |
0.142 |
| Frequency of Optimal Codons |
0.460 |
| Hydropathicity of Protein |
-0.387 |
| Aromaticity Score |
0.066 |
|
