Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
29 |
3.5% |
| Arg[R] |
32 |
3.9% |
| Asn[N] |
54 |
6.6% |
| Asp[D] |
62 |
7.6% |
| Cys[C] |
1 |
0.1% |
| Gln[Q] |
18 |
2.2% |
| Glu[E] |
51 |
6.2% |
| Gly[G] |
55 |
6.7% |
| His[H] |
24 |
2.9% |
| Ile[I] |
45 |
5.5% |
| Leu[L] |
68 |
8.3% |
| Lys[K] |
51 |
6.2% |
| Met[M] |
8 |
1.0% |
| Phe[F] |
45 |
5.5% |
| Pro[P] |
30 |
3.7% |
| Ser[S] |
72 |
8.8% |
| Thr[T] |
45 |
5.5% |
| Trp[W] |
15 |
1.8% |
| Tyr[Y] |
45 |
5.5% |
| Val[V] |
68 |
8.3% |
| Total Length |
818 |
|
| Instability index (II) |
32.89 |
| This classifies the protein as stable |
|
| Carbon |
4230 |
| Hydrogen |
6388 |
| Nitrogen |
1100 |
| Oxygen |
1279 |
| Sulfur |
9 |
| Total number of atoms |
13006 |
| Formula = C4230H6388N1100O1279S9 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
149550 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
149550 M-1 cm-1 |
| assuming all Cys residues are reduced |
149550 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
149550 M-1 cm-1 |
|
|
| Codon Bias |
0.045 |
| Codon Adaptation Index |
0.139 |
| Frequency of Optimal Codons |
0.443 |
| Hydropathicity of Protein |
-0.435 |
| Aromaticity Score |
0.128 |
|
