Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
54 |
7.8% |
| Arg[R] |
26 |
3.8% |
| Asn[N] |
25 |
3.6% |
| Asp[D] |
38 |
5.5% |
| Cys[C] |
9 |
1.3% |
| Gln[Q] |
31 |
4.5% |
| Glu[E] |
56 |
8.1% |
| Gly[G] |
39 |
5.7% |
| His[H] |
13 |
1.9% |
| Ile[I] |
38 |
5.5% |
| Leu[L] |
48 |
7.0% |
| Lys[K] |
60 |
8.7% |
| Met[M] |
14 |
2.0% |
| Phe[F] |
32 |
4.7% |
| Pro[P] |
37 |
5.4% |
| Ser[S] |
47 |
6.8% |
| Thr[T] |
33 |
4.8% |
| Trp[W] |
12 |
1.7% |
| Tyr[Y] |
21 |
3.1% |
| Val[V] |
55 |
8.0% |
| Total Length |
688 |
|
| Instability index (II) |
44.22 |
| This classifies the protein as unstable |
|
| Carbon |
3476 |
| Hydrogen |
5426 |
| Nitrogen |
920 |
| Oxygen |
1034 |
| Sulfur |
23 |
| Total number of atoms |
10879 |
| Formula = C3476H5426N920O1034S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
97790 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
97290 M-1 cm-1 |
| assuming all Cys residues are reduced |
97290 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
97790 M-1 cm-1 |
|
|
| Codon Bias |
0.420 |
| Codon Adaptation Index |
0.355 |
| Frequency of Optimal Codons |
0.659 |
| Hydropathicity of Protein |
-0.394 |
| Aromaticity Score |
0.094 |
|
