Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
32 |
5.1% |
| Arg[R] |
24 |
3.8% |
| Asn[N] |
36 |
5.8% |
| Asp[D] |
34 |
5.4% |
| Cys[C] |
18 |
2.9% |
| Gln[Q] |
31 |
5.0% |
| Glu[E] |
30 |
4.8% |
| Gly[G] |
35 |
5.6% |
| His[H] |
19 |
3.0% |
| Ile[I] |
28 |
4.5% |
| Leu[L] |
55 |
8.8% |
| Lys[K] |
38 |
6.1% |
| Met[M] |
12 |
1.9% |
| Phe[F] |
10 |
1.6% |
| Pro[P] |
45 |
7.2% |
| Ser[S] |
91 |
14.6% |
| Thr[T] |
37 |
5.9% |
| Trp[W] |
4 |
0.6% |
| Tyr[Y] |
19 |
3.0% |
| Val[V] |
27 |
4.3% |
| Total Length |
625 |
|
| Instability index (II) |
67.57 |
| This classifies the protein as unstable |
|
| Carbon |
2935 |
| Hydrogen |
4672 |
| Nitrogen |
844 |
| Oxygen |
968 |
| Sulfur |
30 |
| Total number of atoms |
9449 |
| Formula = C2935H4672N844O968S30 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
51435 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
50310 M-1 cm-1 |
| assuming all Cys residues are reduced |
50310 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
51435 M-1 cm-1 |
|
|
| Codon Bias |
0.147 |
| Codon Adaptation Index |
0.147 |
| Frequency of Optimal Codons |
0.483 |
| Hydropathicity of Protein |
-0.619 |
| Aromaticity Score |
0.053 |
|
