Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
22 |
5.5% |
| Arg[R] |
15 |
3.8% |
| Asn[N] |
31 |
7.8% |
| Asp[D] |
17 |
4.3% |
| Cys[C] |
10 |
2.5% |
| Gln[Q] |
20 |
5.0% |
| Glu[E] |
17 |
4.3% |
| Gly[G] |
13 |
3.3% |
| His[H] |
11 |
2.8% |
| Ile[I] |
34 |
8.5% |
| Leu[L] |
44 |
11.1% |
| Lys[K] |
36 |
9.0% |
| Met[M] |
7 |
1.8% |
| Phe[F] |
20 |
5.0% |
| Pro[P] |
18 |
4.5% |
| Ser[S] |
26 |
6.5% |
| Thr[T] |
24 |
6.0% |
| Trp[W] |
2 |
0.5% |
| Tyr[Y] |
12 |
3.0% |
| Val[V] |
19 |
4.8% |
| Total Length |
398 |
|
| Instability index (II) |
31.22 |
| This classifies the protein as stable |
|
| Carbon |
2043 |
| Hydrogen |
3264 |
| Nitrogen |
554 |
| Oxygen |
580 |
| Sulfur |
17 |
| Total number of atoms |
6458 |
| Formula = C2043H3264N554O580S17 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
29505 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
28880 M-1 cm-1 |
| assuming all Cys residues are reduced |
28880 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
29505 M-1 cm-1 |
|
|
| Codon Bias |
0.120 |
| Codon Adaptation Index |
0.162 |
| Frequency of Optimal Codons |
0.488 |
| Hydropathicity of Protein |
-0.240 |
| Aromaticity Score |
0.085 |
|
