DUR3/YHL016C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 72 9.8%
Arg[R] 17 2.3%
Asn[N] 23 3.1%
Asp[D] 28 3.8%
Cys[C] 11 1.5%
Gln[Q] 20 2.7%
Glu[E] 32 4.4%
Gly[G] 55 7.5%
His[H] 9 1.2%
Ile[I] 57 7.8%
Leu[L] 76 10.3%
Lys[K] 32 4.4%
Met[M] 26 3.5%
Phe[F] 39 5.3%
Pro[P] 27 3.7%
Ser[S] 53 7.2%
Thr[T] 49 6.7%
Trp[W] 12 1.6%
Tyr[Y] 38 5.2%
Val[V] 59 8.0%
Total Length 735

Instability index
Instability index (II) 31.94
This classifies the protein as stable

Atomic composition
Carbon 3709
Hydrogen 5733
Nitrogen 891
Oxygen 1039
Sulfur 37
Total number of atoms 11409
Formula = C3709H5733N891O1039S37

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 123245 M-1 cm-1
assuming NO Cys residues appear as half cystines 122620 M-1 cm-1
assuming all Cys residues are reduced 122620 M-1 cm-1
assuming all pairs of Cys residues form cystines 123245 M-1 cm-1

Aliphatic index
Aliphatic index 103.65

Coding Region Translation Calculations
Codon Bias 0.123
Codon Adaptation Index 0.155
Frequency of Optimal Codons 0.475
Hydropathicity of Protein 0.430
Aromaticity Score 0.121