Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
54 |
5.8% |
| Arg[R] |
30 |
3.2% |
| Asn[N] |
72 |
7.7% |
| Asp[D] |
55 |
5.9% |
| Cys[C] |
7 |
0.7% |
| Gln[Q] |
30 |
3.2% |
| Glu[E] |
57 |
6.1% |
| Gly[G] |
45 |
4.8% |
| His[H] |
21 |
2.2% |
| Ile[I] |
49 |
5.2% |
| Leu[L] |
62 |
6.6% |
| Lys[K] |
80 |
8.5% |
| Met[M] |
23 |
2.4% |
| Phe[F] |
17 |
1.8% |
| Pro[P] |
67 |
7.1% |
| Ser[S] |
120 |
12.8% |
| Thr[T] |
78 |
8.3% |
| Trp[W] |
5 |
0.5% |
| Tyr[Y] |
18 |
1.9% |
| Val[V] |
49 |
5.2% |
| Total Length |
939 |
|
| Instability index (II) |
48.76 |
| This classifies the protein as unstable |
|
| Carbon |
4405 |
| Hydrogen |
7104 |
| Nitrogen |
1258 |
| Oxygen |
1482 |
| Sulfur |
30 |
| Total number of atoms |
14279 |
| Formula = C4405H7104N1258O1482S30 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
54695 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
54320 M-1 cm-1 |
| assuming all Cys residues are reduced |
54320 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
54695 M-1 cm-1 |
|
|
| Codon Bias |
0.094 |
| Codon Adaptation Index |
0.152 |
| Frequency of Optimal Codons |
0.463 |
| Hydropathicity of Protein |
-0.744 |
| Aromaticity Score |
0.043 |
|
