Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
79 |
5.4% |
| Arg[R] |
87 |
5.9% |
| Asn[N] |
87 |
5.9% |
| Asp[D] |
111 |
7.6% |
| Cys[C] |
6 |
0.4% |
| Gln[Q] |
52 |
3.5% |
| Glu[E] |
131 |
8.9% |
| Gly[G] |
76 |
5.2% |
| His[H] |
31 |
2.1% |
| Ile[I] |
79 |
5.4% |
| Leu[L] |
133 |
9.1% |
| Lys[K] |
138 |
9.4% |
| Met[M] |
34 |
2.3% |
| Phe[F] |
48 |
3.3% |
| Pro[P] |
59 |
4.0% |
| Ser[S] |
118 |
8.0% |
| Thr[T] |
76 |
5.2% |
| Trp[W] |
18 |
1.2% |
| Tyr[Y] |
45 |
3.1% |
| Val[V] |
60 |
4.1% |
| Total Length |
1468 |
|
| Instability index (II) |
44.88 |
| This classifies the protein as unstable |
|
| Carbon |
7380 |
| Hydrogen |
11712 |
| Nitrogen |
2086 |
| Oxygen |
2331 |
| Sulfur |
40 |
| Total number of atoms |
23549 |
| Formula = C7380H11712N2086O2331S40 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
166425 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
166050 M-1 cm-1 |
| assuming all Cys residues are reduced |
166050 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
166425 M-1 cm-1 |
|
|
| Codon Bias |
0.097 |
| Codon Adaptation Index |
0.170 |
| Frequency of Optimal Codons |
0.463 |
| Hydropathicity of Protein |
-0.845 |
| Aromaticity Score |
0.076 |
|
