Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
52 |
5.7% |
| Arg[R] |
23 |
2.5% |
| Asn[N] |
74 |
8.1% |
| Asp[D] |
48 |
5.3% |
| Cys[C] |
6 |
0.7% |
| Gln[Q] |
38 |
4.2% |
| Glu[E] |
61 |
6.7% |
| Gly[G] |
43 |
4.7% |
| His[H] |
29 |
3.2% |
| Ile[I] |
56 |
6.1% |
| Leu[L] |
57 |
6.2% |
| Lys[K] |
87 |
9.5% |
| Met[M] |
20 |
2.2% |
| Phe[F] |
27 |
3.0% |
| Pro[P] |
48 |
5.3% |
| Ser[S] |
107 |
11.7% |
| Thr[T] |
56 |
6.1% |
| Trp[W] |
5 |
0.5% |
| Tyr[Y] |
36 |
3.9% |
| Val[V] |
39 |
4.3% |
| Total Length |
912 |
|
| Instability index (II) |
48.38 |
| This classifies the protein as unstable |
|
| Carbon |
4457 |
| Hydrogen |
7013 |
| Nitrogen |
1243 |
| Oxygen |
1442 |
| Sulfur |
26 |
| Total number of atoms |
14181 |
| Formula = C4457H7013N1243O1442S26 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
81515 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
81140 M-1 cm-1 |
| assuming all Cys residues are reduced |
81140 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
81515 M-1 cm-1 |
|
|
| Codon Bias |
0.125 |
| Codon Adaptation Index |
0.179 |
| Frequency of Optimal Codons |
0.493 |
| Hydropathicity of Protein |
-0.795 |
| Aromaticity Score |
0.075 |
|
