Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
45 |
3.9% |
| Arg[R] |
66 |
5.8% |
| Asn[N] |
79 |
6.9% |
| Asp[D] |
74 |
6.5% |
| Cys[C] |
15 |
1.3% |
| Gln[Q] |
35 |
3.1% |
| Glu[E] |
70 |
6.1% |
| Gly[G] |
56 |
4.9% |
| His[H] |
29 |
2.5% |
| Ile[I] |
57 |
5.0% |
| Leu[L] |
102 |
8.9% |
| Lys[K] |
93 |
8.1% |
| Met[M] |
20 |
1.8% |
| Phe[F] |
39 |
3.4% |
| Pro[P] |
58 |
5.1% |
| Ser[S] |
166 |
14.5% |
| Thr[T] |
59 |
5.2% |
| Trp[W] |
3 |
0.3% |
| Tyr[Y] |
18 |
1.6% |
| Val[V] |
58 |
5.1% |
| Total Length |
1142 |
|
| Instability index (II) |
52.63 |
| This classifies the protein as unstable |
|
| Carbon |
5471 |
| Hydrogen |
8838 |
| Nitrogen |
1608 |
| Oxygen |
1788 |
| Sulfur |
35 |
| Total number of atoms |
17740 |
| Formula = C5471H8838N1608O1788S35 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
44195 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
43320 M-1 cm-1 |
| assuming all Cys residues are reduced |
43320 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
44195 M-1 cm-1 |
|
|
| Codon Bias |
-0.020 |
| Codon Adaptation Index |
0.115 |
| Frequency of Optimal Codons |
0.386 |
| Hydropathicity of Protein |
-0.716 |
| Aromaticity Score |
0.053 |
|
