Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
38 |
4.7% |
| Arg[R] |
58 |
7.2% |
| Asn[N] |
40 |
4.9% |
| Asp[D] |
53 |
6.6% |
| Cys[C] |
4 |
0.5% |
| Gln[Q] |
41 |
5.1% |
| Glu[E] |
51 |
6.3% |
| Gly[G] |
58 |
7.2% |
| His[H] |
17 |
2.1% |
| Ile[I] |
32 |
4.0% |
| Leu[L] |
57 |
7.0% |
| Lys[K] |
39 |
4.8% |
| Met[M] |
12 |
1.5% |
| Phe[F] |
41 |
5.1% |
| Pro[P] |
37 |
4.6% |
| Ser[S] |
63 |
7.8% |
| Thr[T] |
74 |
9.1% |
| Trp[W] |
13 |
1.6% |
| Tyr[Y] |
28 |
3.5% |
| Val[V] |
53 |
6.6% |
| Total Length |
809 |
|
| Instability index (II) |
40.11 |
| This classifies the protein as unstable |
|
| Carbon |
4051 |
| Hydrogen |
6266 |
| Nitrogen |
1150 |
| Oxygen |
1264 |
| Sulfur |
16 |
| Total number of atoms |
12747 |
| Formula = C4051H6266N1150O1264S16 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
113470 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
113220 M-1 cm-1 |
| assuming all Cys residues are reduced |
113220 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
113470 M-1 cm-1 |
|
|
| Codon Bias |
0.162 |
| Codon Adaptation Index |
0.186 |
| Frequency of Optimal Codons |
0.499 |
| Hydropathicity of Protein |
-0.678 |
| Aromaticity Score |
0.101 |
|
