Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
41 |
3.8% |
| Arg[R] |
30 |
2.7% |
| Asn[N] |
148 |
13.5% |
| Asp[D] |
54 |
4.9% |
| Cys[C] |
7 |
0.6% |
| Gln[Q] |
73 |
6.7% |
| Glu[E] |
56 |
5.1% |
| Gly[G] |
24 |
2.2% |
| His[H] |
23 |
2.1% |
| Ile[I] |
88 |
8.1% |
| Leu[L] |
80 |
7.3% |
| Lys[K] |
92 |
8.4% |
| Met[M] |
26 |
2.4% |
| Phe[F] |
33 |
3.0% |
| Pro[P] |
54 |
4.9% |
| Ser[S] |
125 |
11.4% |
| Thr[T] |
69 |
6.3% |
| Trp[W] |
4 |
0.4% |
| Tyr[Y] |
28 |
2.6% |
| Val[V] |
38 |
3.5% |
| Total Length |
1093 |
|
| Instability index (II) |
53.38 |
| This classifies the protein as unstable |
|
| Carbon |
5357 |
| Hydrogen |
8572 |
| Nitrogen |
1546 |
| Oxygen |
1757 |
| Sulfur |
33 |
| Total number of atoms |
17265 |
| Formula = C5357H8572N1546O1757S33 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
64095 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
63720 M-1 cm-1 |
| assuming all Cys residues are reduced |
63720 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
64095 M-1 cm-1 |
|
|
| Codon Bias |
0.053 |
| Codon Adaptation Index |
0.145 |
| Frequency of Optimal Codons |
0.462 |
| Hydropathicity of Protein |
-0.839 |
| Aromaticity Score |
0.059 |
|
