NUP157/YER105C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 76 5.5%
Arg[R] 63 4.5%
Asn[N] 87 6.3%
Asp[D] 83 6.0%
Cys[C] 21 1.5%
Gln[Q] 37 2.7%
Glu[E] 86 6.2%
Gly[G] 65 4.7%
His[H] 24 1.7%
Ile[I] 108 7.8%
Leu[L] 148 10.6%
Lys[K] 92 6.6%
Met[M] 14 1.0%
Phe[F] 71 5.1%
Pro[P] 51 3.7%
Ser[S] 144 10.4%
Thr[T] 74 5.3%
Trp[W] 9 0.6%
Tyr[Y] 55 4.0%
Val[V] 83 6.0%
Total Length 1391

Instability index
Instability index (II) 39.39
This classifies the protein as stable

Atomic composition
Carbon 7027
Hydrogen 11053
Nitrogen 1853
Oxygen 2127
Sulfur 35
Total number of atoms 22095
Formula = C7027H11053N1853O2127S35

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 132700 M-1 cm-1
assuming NO Cys residues appear as half cystines 131450 M-1 cm-1
assuming all Cys residues are reduced 131450 M-1 cm-1
assuming all pairs of Cys residues form cystines 132700 M-1 cm-1

Aliphatic index
Aliphatic index 94.54

Coding Region Translation Calculations
Codon Bias 0.026
Codon Adaptation Index 0.133
Frequency of Optimal Codons 0.425
Hydropathicity of Protein -0.206
Aromaticity Score 0.097