RPS8B/YER102W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 23 11.5%
Arg[R] 22 11.0%
Asn[N] 8 4.0%
Asp[D] 3 1.5%
Cys[C] 2 1.0%
Gln[Q] 7 3.5%
Glu[E] 14 7.0%
Gly[G] 16 8.0%
His[H] 4 2.0%
Ile[I] 11 5.5%
Leu[L] 10 5.0%
Lys[K] 22 11.0%
Met[M] 1 0.5%
Phe[F] 7 3.5%
Pro[P] 4 2.0%
Ser[S] 17 8.5%
Thr[T] 12 6.0%
Trp[W] 3 1.5%
Tyr[Y] 6 3.0%
Val[V] 8 4.0%
Total Length 200

Instability index
Instability index (II) 52.34
This classifies the protein as unstable

Atomic composition
Carbon 984
Hydrogen 1604
Nitrogen 314
Oxygen 285
Sulfur 3
Total number of atoms 3190
Formula = C984H1604N314O285S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 25565 M-1 cm-1
assuming NO Cys residues appear as half cystines 25440 M-1 cm-1
assuming all Cys residues are reduced 25440 M-1 cm-1
assuming all pairs of Cys residues form cystines 25565 M-1 cm-1

Aliphatic index
Aliphatic index 64.05

Coding Region Translation Calculations
Codon Bias 0.751
Codon Adaptation Index 0.718
Frequency of Optimal Codons 0.847
Hydropathicity of Protein -0.830
Aromaticity Score 0.080