Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
39 |
9.8% |
| Arg[R] |
20 |
5.0% |
| Asn[N] |
13 |
3.2% |
| Asp[D] |
27 |
6.8% |
| Cys[C] |
8 |
2.0% |
| Gln[Q] |
21 |
5.2% |
| Glu[E] |
30 |
7.5% |
| Gly[G] |
41 |
10.2% |
| His[H] |
7 |
1.8% |
| Ile[I] |
16 |
4.0% |
| Leu[L] |
38 |
9.5% |
| Lys[K] |
17 |
4.2% |
| Met[M] |
13 |
3.2% |
| Phe[F] |
14 |
3.5% |
| Pro[P] |
13 |
3.2% |
| Ser[S] |
27 |
6.8% |
| Thr[T] |
19 |
4.8% |
| Trp[W] |
0 |
0.0% |
| Tyr[Y] |
8 |
2.0% |
| Val[V] |
29 |
7.2% |
| Total Length |
400 |
|
| Instability index (II) |
37.37 |
| This classifies the protein as stable |
|
| Carbon |
1856 |
| Hydrogen |
2973 |
| Nitrogen |
525 |
| Oxygen |
603 |
| Sulfur |
21 |
| Total number of atoms |
5978 |
| Formula = C1856H2973N525O603S21 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
12420 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
11920 M-1 cm-1 |
| assuming all Cys residues are reduced |
11920 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
12420 M-1 cm-1 |
|
|
| Codon Bias |
0.214 |
| Codon Adaptation Index |
0.173 |
| Frequency of Optimal Codons |
0.517 |
| Hydropathicity of Protein |
-0.218 |
| Aromaticity Score |
0.055 |
|
