Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
77 |
8.7% |
| Arg[R] |
42 |
4.7% |
| Asn[N] |
43 |
4.8% |
| Asp[D] |
50 |
5.6% |
| Cys[C] |
14 |
1.6% |
| Gln[Q] |
32 |
3.6% |
| Glu[E] |
58 |
6.5% |
| Gly[G] |
50 |
5.6% |
| His[H] |
17 |
1.9% |
| Ile[I] |
59 |
6.6% |
| Leu[L] |
69 |
7.8% |
| Lys[K] |
53 |
6.0% |
| Met[M] |
27 |
3.0% |
| Phe[F] |
31 |
3.5% |
| Pro[P] |
43 |
4.8% |
| Ser[S] |
64 |
7.2% |
| Thr[T] |
58 |
6.5% |
| Trp[W] |
10 |
1.1% |
| Tyr[Y] |
43 |
4.8% |
| Val[V] |
48 |
5.4% |
| Total Length |
888 |
|
| Instability index (II) |
42.12 |
| This classifies the protein as unstable |
|
| Carbon |
4425 |
| Hydrogen |
6912 |
| Nitrogen |
1186 |
| Oxygen |
1345 |
| Sulfur |
41 |
| Total number of atoms |
13909 |
| Formula = C4425H6912N1186O1345S41 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
119945 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
119070 M-1 cm-1 |
| assuming all Cys residues are reduced |
119070 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
119945 M-1 cm-1 |
|
|
| Codon Bias |
0.332 |
| Codon Adaptation Index |
0.312 |
| Frequency of Optimal Codons |
0.603 |
| Hydropathicity of Protein |
-0.332 |
| Aromaticity Score |
0.095 |
|
