Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
67 |
7.8% |
| Arg[R] |
36 |
4.2% |
| Asn[N] |
48 |
5.6% |
| Asp[D] |
45 |
5.2% |
| Cys[C] |
8 |
0.9% |
| Gln[Q] |
28 |
3.2% |
| Glu[E] |
48 |
5.6% |
| Gly[G] |
62 |
7.2% |
| His[H] |
15 |
1.7% |
| Ile[I] |
64 |
7.4% |
| Leu[L] |
79 |
9.2% |
| Lys[K] |
62 |
7.2% |
| Met[M] |
9 |
1.0% |
| Phe[F] |
30 |
3.5% |
| Pro[P] |
34 |
3.9% |
| Ser[S] |
76 |
8.8% |
| Thr[T] |
42 |
4.9% |
| Trp[W] |
8 |
0.9% |
| Tyr[Y] |
33 |
3.8% |
| Val[V] |
69 |
8.0% |
| Total Length |
863 |
|
| Instability index (II) |
31.66 |
| This classifies the protein as stable |
|
| Carbon |
4248 |
| Hydrogen |
6751 |
| Nitrogen |
1147 |
| Oxygen |
1277 |
| Sulfur |
17 |
| Total number of atoms |
13440 |
| Formula = C4248H6751N1147O1277S17 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
93670 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
93170 M-1 cm-1 |
| assuming all Cys residues are reduced |
93170 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
93670 M-1 cm-1 |
|
|
| Codon Bias |
0.201 |
| Codon Adaptation Index |
0.198 |
| Frequency of Optimal Codons |
0.524 |
| Hydropathicity of Protein |
-0.166 |
| Aromaticity Score |
0.082 |
|
