Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
33 |
5.6% |
| Arg[R] |
24 |
4.1% |
| Asn[N] |
50 |
8.5% |
| Asp[D] |
26 |
4.4% |
| Cys[C] |
12 |
2.0% |
| Gln[Q] |
30 |
5.1% |
| Glu[E] |
32 |
5.5% |
| Gly[G] |
31 |
5.3% |
| His[H] |
21 |
3.6% |
| Ile[I] |
34 |
5.8% |
| Leu[L] |
39 |
6.6% |
| Lys[K] |
38 |
6.5% |
| Met[M] |
8 |
1.4% |
| Phe[F] |
15 |
2.6% |
| Pro[P] |
41 |
7.0% |
| Ser[S] |
50 |
8.5% |
| Thr[T] |
51 |
8.7% |
| Trp[W] |
2 |
0.3% |
| Tyr[Y] |
23 |
3.9% |
| Val[V] |
27 |
4.6% |
| Total Length |
587 |
|
| Instability index (II) |
43.81 |
| This classifies the protein as unstable |
|
| Carbon |
2845 |
| Hydrogen |
4479 |
| Nitrogen |
821 |
| Oxygen |
908 |
| Sulfur |
20 |
| Total number of atoms |
9073 |
| Formula = C2845H4479N821O908S20 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
46020 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
45270 M-1 cm-1 |
| assuming all Cys residues are reduced |
45270 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
46020 M-1 cm-1 |
|
|
| Codon Bias |
0.075 |
| Codon Adaptation Index |
0.151 |
| Frequency of Optimal Codons |
0.458 |
| Hydropathicity of Protein |
-0.734 |
| Aromaticity Score |
0.068 |
|
