Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
27 |
5.3% |
| Arg[R] |
26 |
5.1% |
| Asn[N] |
26 |
5.1% |
| Asp[D] |
25 |
5.0% |
| Cys[C] |
4 |
0.8% |
| Gln[Q] |
22 |
4.4% |
| Glu[E] |
34 |
6.7% |
| Gly[G] |
15 |
3.0% |
| His[H] |
12 |
2.4% |
| Ile[I] |
35 |
6.9% |
| Leu[L] |
52 |
10.3% |
| Lys[K] |
44 |
8.7% |
| Met[M] |
8 |
1.6% |
| Phe[F] |
17 |
3.4% |
| Pro[P] |
28 |
5.5% |
| Ser[S] |
68 |
13.5% |
| Thr[T] |
30 |
5.9% |
| Trp[W] |
1 |
0.2% |
| Tyr[Y] |
11 |
2.2% |
| Val[V] |
20 |
4.0% |
| Total Length |
505 |
|
| Instability index (II) |
55.61 |
| This classifies the protein as unstable |
|
| Carbon |
2488 |
| Hydrogen |
4038 |
| Nitrogen |
700 |
| Oxygen |
781 |
| Sulfur |
12 |
| Total number of atoms |
8019 |
| Formula = C2488H4038N700O781S12 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
22140 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
21890 M-1 cm-1 |
| assuming all Cys residues are reduced |
21890 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
22140 M-1 cm-1 |
|
|
| Codon Bias |
0.054 |
| Codon Adaptation Index |
0.133 |
| Frequency of Optimal Codons |
0.433 |
| Hydropathicity of Protein |
-0.559 |
| Aromaticity Score |
0.057 |
|
