Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
58 |
10.9% |
| Arg[R] |
11 |
2.1% |
| Asn[N] |
27 |
5.1% |
| Asp[D] |
15 |
2.8% |
| Cys[C] |
7 |
1.3% |
| Gln[Q] |
10 |
1.9% |
| Glu[E] |
24 |
4.5% |
| Gly[G] |
51 |
9.6% |
| His[H] |
5 |
0.9% |
| Ile[I] |
45 |
8.4% |
| Leu[L] |
46 |
8.6% |
| Lys[K] |
18 |
3.4% |
| Met[M] |
16 |
3.0% |
| Phe[F] |
39 |
7.3% |
| Pro[P] |
15 |
2.8% |
| Ser[S] |
41 |
7.7% |
| Thr[T] |
26 |
4.9% |
| Trp[W] |
15 |
2.8% |
| Tyr[Y] |
25 |
4.7% |
| Val[V] |
39 |
7.3% |
| Total Length |
533 |
|
| Instability index (II) |
30.39 |
| This classifies the protein as stable |
|
| Carbon |
2703 |
| Hydrogen |
4062 |
| Nitrogen |
646 |
| Oxygen |
741 |
| Sulfur |
23 |
| Total number of atoms |
8175 |
| Formula = C2703H4062N646O741S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
120125 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
119750 M-1 cm-1 |
| assuming all Cys residues are reduced |
119750 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
120125 M-1 cm-1 |
|
|
| Codon Bias |
0.238 |
| Codon Adaptation Index |
0.202 |
| Frequency of Optimal Codons |
0.544 |
| Hydropathicity of Protein |
0.487 |
| Aromaticity Score |
0.148 |
|
