Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
24 |
4.4% |
| Arg[R] |
28 |
5.1% |
| Asn[N] |
56 |
10.2% |
| Asp[D] |
35 |
6.4% |
| Cys[C] |
5 |
0.9% |
| Gln[Q] |
15 |
2.7% |
| Glu[E] |
35 |
6.4% |
| Gly[G] |
30 |
5.5% |
| His[H] |
13 |
2.4% |
| Ile[I] |
26 |
4.7% |
| Leu[L] |
37 |
6.8% |
| Lys[K] |
50 |
9.1% |
| Met[M] |
9 |
1.6% |
| Phe[F] |
26 |
4.7% |
| Pro[P] |
33 |
6.0% |
| Ser[S] |
47 |
8.6% |
| Thr[T] |
25 |
4.6% |
| Trp[W] |
4 |
0.7% |
| Tyr[Y] |
20 |
3.6% |
| Val[V] |
30 |
5.5% |
| Total Length |
548 |
|
| Instability index (II) |
42.69 |
| This classifies the protein as unstable |
|
| Carbon |
2744 |
| Hydrogen |
4279 |
| Nitrogen |
783 |
| Oxygen |
852 |
| Sulfur |
14 |
| Total number of atoms |
8672 |
| Formula = C2744H4279N783O852S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
52050 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
51800 M-1 cm-1 |
| assuming all Cys residues are reduced |
51800 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
52050 M-1 cm-1 |
|
|
| Codon Bias |
0.001 |
| Codon Adaptation Index |
0.136 |
| Frequency of Optimal Codons |
0.413 |
| Hydropathicity of Protein |
-0.869 |
| Aromaticity Score |
0.091 |
|
