Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
21 |
3.1% |
| Arg[R] |
27 |
3.9% |
| Asn[N] |
50 |
7.3% |
| Asp[D] |
55 |
8.0% |
| Cys[C] |
2 |
0.3% |
| Gln[Q] |
20 |
2.9% |
| Glu[E] |
63 |
9.2% |
| Gly[G] |
24 |
3.5% |
| His[H] |
15 |
2.2% |
| Ile[I] |
59 |
8.6% |
| Leu[L] |
77 |
11.2% |
| Lys[K] |
65 |
9.5% |
| Met[M] |
14 |
2.0% |
| Phe[F] |
24 |
3.5% |
| Pro[P] |
26 |
3.8% |
| Ser[S] |
66 |
9.6% |
| Thr[T] |
28 |
4.1% |
| Trp[W] |
2 |
0.3% |
| Tyr[Y] |
15 |
2.2% |
| Val[V] |
34 |
4.9% |
| Total Length |
687 |
|
| Instability index (II) |
50.44 |
| This classifies the protein as unstable |
|
| Carbon |
3463 |
| Hydrogen |
5581 |
| Nitrogen |
935 |
| Oxygen |
1103 |
| Sulfur |
16 |
| Total number of atoms |
11098 |
| Formula = C3463H5581N935O1103S16 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
33475 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
33350 M-1 cm-1 |
| assuming all Cys residues are reduced |
33350 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
33475 M-1 cm-1 |
|
|
| Codon Bias |
0.031 |
| Codon Adaptation Index |
0.151 |
| Frequency of Optimal Codons |
0.438 |
| Hydropathicity of Protein |
-0.565 |
| Aromaticity Score |
0.060 |
|
