Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
54 |
7.1% |
| Arg[R] |
47 |
6.2% |
| Asn[N] |
46 |
6.0% |
| Asp[D] |
41 |
5.4% |
| Cys[C] |
4 |
0.5% |
| Gln[Q] |
23 |
3.0% |
| Glu[E] |
46 |
6.0% |
| Gly[G] |
60 |
7.9% |
| His[H] |
15 |
2.0% |
| Ile[I] |
47 |
6.2% |
| Leu[L] |
71 |
9.3% |
| Lys[K] |
53 |
7.0% |
| Met[M] |
19 |
2.5% |
| Phe[F] |
41 |
5.4% |
| Pro[P] |
38 |
5.0% |
| Ser[S] |
50 |
6.6% |
| Thr[T] |
45 |
5.9% |
| Trp[W] |
3 |
0.4% |
| Tyr[Y] |
16 |
2.1% |
| Val[V] |
42 |
5.5% |
| Total Length |
761 |
|
| Instability index (II) |
36.36 |
| This classifies the protein as stable |
|
| Carbon |
3756 |
| Hydrogen |
5985 |
| Nitrogen |
1057 |
| Oxygen |
1116 |
| Sulfur |
23 |
| Total number of atoms |
11937 |
| Formula = C3756H5985N1057O1116S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
40590 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
40340 M-1 cm-1 |
| assuming all Cys residues are reduced |
40340 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
40590 M-1 cm-1 |
|
|
| Codon Bias |
0.115 |
| Codon Adaptation Index |
0.140 |
| Frequency of Optimal Codons |
0.464 |
| Hydropathicity of Protein |
-0.363 |
| Aromaticity Score |
0.079 |
|
