Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
34 |
6.9% |
| Arg[R] |
17 |
3.4% |
| Asn[N] |
32 |
6.5% |
| Asp[D] |
35 |
7.1% |
| Cys[C] |
6 |
1.2% |
| Gln[Q] |
12 |
2.4% |
| Glu[E] |
26 |
5.2% |
| Gly[G] |
36 |
7.3% |
| His[H] |
14 |
2.8% |
| Ile[I] |
30 |
6.0% |
| Leu[L] |
47 |
9.5% |
| Lys[K] |
40 |
8.1% |
| Met[M] |
11 |
2.2% |
| Phe[F] |
24 |
4.8% |
| Pro[P] |
20 |
4.0% |
| Ser[S] |
34 |
6.9% |
| Thr[T] |
20 |
4.0% |
| Trp[W] |
3 |
0.6% |
| Tyr[Y] |
19 |
3.8% |
| Val[V] |
36 |
7.3% |
| Total Length |
496 |
|
| Instability index (II) |
41.71 |
| This classifies the protein as unstable |
|
| Carbon |
2475 |
| Hydrogen |
3874 |
| Nitrogen |
662 |
| Oxygen |
736 |
| Sulfur |
17 |
| Total number of atoms |
7764 |
| Formula = C2475H3874N662O736S17 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
45185 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
44810 M-1 cm-1 |
| assuming all Cys residues are reduced |
44810 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
45185 M-1 cm-1 |
|
|
| Codon Bias |
0.215 |
| Codon Adaptation Index |
0.220 |
| Frequency of Optimal Codons |
0.535 |
| Hydropathicity of Protein |
-0.264 |
| Aromaticity Score |
0.093 |
|
