HXT13/YEL069C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 36 6.4%
Arg[R] 19 3.4%
Asn[N] 17 3.0%
Asp[D] 21 3.7%
Cys[C] 14 2.5%
Gln[Q] 13 2.3%
Glu[E] 27 4.8%
Gly[G] 53 9.4%
His[H] 6 1.1%
Ile[I] 48 8.5%
Leu[L] 53 9.4%
Lys[K] 29 5.1%
Met[M] 16 2.8%
Phe[F] 40 7.1%
Pro[P] 22 3.9%
Ser[S] 44 7.8%
Thr[T] 25 4.4%
Trp[W] 13 2.3%
Tyr[Y] 25 4.4%
Val[V] 43 7.6%
Total Length 564

Instability index
Instability index (II) 36.57
This classifies the protein as stable

Atomic composition
Carbon 2903
Hydrogen 4435
Nitrogen 705
Oxygen 785
Sulfur 30
Total number of atoms 8858
Formula = C2903H4435N705O785S30

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 109625 M-1 cm-1
assuming NO Cys residues appear as half cystines 108750 M-1 cm-1
assuming all Cys residues are reduced 108750 M-1 cm-1
assuming all pairs of Cys residues form cystines 109625 M-1 cm-1

Aliphatic index
Aliphatic index 98.33

Coding Region Translation Calculations
Codon Bias 0.231
Codon Adaptation Index 0.193
Frequency of Optimal Codons 0.542
Hydropathicity of Protein 0.348
Aromaticity Score 0.138