Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
47 |
7.5% |
| Arg[R] |
22 |
3.5% |
| Asn[N] |
20 |
3.2% |
| Asp[D] |
19 |
3.0% |
| Cys[C] |
11 |
1.8% |
| Gln[Q] |
11 |
1.8% |
| Glu[E] |
28 |
4.5% |
| Gly[G] |
42 |
6.7% |
| His[H] |
14 |
2.2% |
| Ile[I] |
69 |
11.0% |
| Leu[L] |
64 |
10.2% |
| Lys[K] |
29 |
4.6% |
| Met[M] |
15 |
2.4% |
| Phe[F] |
45 |
7.2% |
| Pro[P] |
24 |
3.8% |
| Ser[S] |
47 |
7.5% |
| Thr[T] |
38 |
6.1% |
| Trp[W] |
15 |
2.4% |
| Tyr[Y] |
26 |
4.1% |
| Val[V] |
42 |
6.7% |
| Total Length |
628 |
|
| Instability index (II) |
36.67 |
| This classifies the protein as stable |
|
| Carbon |
3299 |
| Hydrogen |
5061 |
| Nitrogen |
797 |
| Oxygen |
865 |
| Sulfur |
26 |
| Total number of atoms |
10048 |
| Formula = C3299H5061N797O865S26 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
121865 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
121240 M-1 cm-1 |
| assuming all Cys residues are reduced |
121240 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
121865 M-1 cm-1 |
|
|
| Codon Bias |
0.231 |
| Codon Adaptation Index |
0.191 |
| Frequency of Optimal Codons |
0.547 |
| Hydropathicity of Protein |
0.478 |
| Aromaticity Score |
0.137 |
|
