Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
93 |
7.7% |
| Arg[R] |
44 |
3.6% |
| Asn[N] |
46 |
3.8% |
| Asp[D] |
61 |
5.0% |
| Cys[C] |
13 |
1.1% |
| Gln[Q] |
41 |
3.4% |
| Glu[E] |
75 |
6.2% |
| Gly[G] |
72 |
5.9% |
| His[H] |
21 |
1.7% |
| Ile[I] |
83 |
6.8% |
| Leu[L] |
136 |
11.2% |
| Lys[K] |
82 |
6.7% |
| Met[M] |
31 |
2.6% |
| Phe[F] |
69 |
5.7% |
| Pro[P] |
63 |
5.2% |
| Ser[S] |
89 |
7.3% |
| Thr[T] |
68 |
5.6% |
| Trp[W] |
12 |
1.0% |
| Tyr[Y] |
35 |
2.9% |
| Val[V] |
81 |
6.7% |
| Total Length |
1215 |
|
| Instability index (II) |
35.94 |
| This classifies the protein as stable |
|
| Carbon |
6148 |
| Hydrogen |
9676 |
| Nitrogen |
1570 |
| Oxygen |
1767 |
| Sulfur |
44 |
| Total number of atoms |
19205 |
| Formula = C6148H9676N1570O1767S44 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
118900 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
118150 M-1 cm-1 |
| assuming all Cys residues are reduced |
118150 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
118900 M-1 cm-1 |
|
|
| Codon Bias |
0.278 |
| Codon Adaptation Index |
0.257 |
| Frequency of Optimal Codons |
0.567 |
| Hydropathicity of Protein |
0.010 |
| Aromaticity Score |
0.095 |
|
