Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
30 |
5.4% |
| Arg[R] |
22 |
4.0% |
| Asn[N] |
37 |
6.7% |
| Asp[D] |
45 |
8.2% |
| Cys[C] |
7 |
1.3% |
| Gln[Q] |
50 |
9.1% |
| Glu[E] |
22 |
4.0% |
| Gly[G] |
33 |
6.0% |
| His[H] |
8 |
1.5% |
| Ile[I] |
40 |
7.3% |
| Leu[L] |
52 |
9.4% |
| Lys[K] |
40 |
7.3% |
| Met[M] |
10 |
1.8% |
| Phe[F] |
19 |
3.4% |
| Pro[P] |
17 |
3.1% |
| Ser[S] |
52 |
9.4% |
| Thr[T] |
27 |
4.9% |
| Trp[W] |
5 |
0.9% |
| Tyr[Y] |
7 |
1.3% |
| Val[V] |
28 |
5.1% |
| Total Length |
551 |
|
| Instability index (II) |
47.82 |
| This classifies the protein as unstable |
|
| Carbon |
2665 |
| Hydrogen |
4293 |
| Nitrogen |
765 |
| Oxygen |
859 |
| Sulfur |
17 |
| Total number of atoms |
8599 |
| Formula = C2665H4293N765O859S17 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
38305 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
37930 M-1 cm-1 |
| assuming all Cys residues are reduced |
37930 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
38305 M-1 cm-1 |
|
|
| Codon Bias |
0.075 |
| Codon Adaptation Index |
0.153 |
| Frequency of Optimal Codons |
0.457 |
| Hydropathicity of Protein |
-0.536 |
| Aromaticity Score |
0.056 |
|
