Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
16 |
4.4% |
| Arg[R] |
18 |
5.0% |
| Asn[N] |
32 |
8.8% |
| Asp[D] |
20 |
5.5% |
| Cys[C] |
4 |
1.1% |
| Gln[Q] |
10 |
2.8% |
| Glu[E] |
28 |
7.7% |
| Gly[G] |
16 |
4.4% |
| His[H] |
6 |
1.7% |
| Ile[I] |
27 |
7.5% |
| Leu[L] |
37 |
10.2% |
| Lys[K] |
34 |
9.4% |
| Met[M] |
3 |
0.8% |
| Phe[F] |
18 |
5.0% |
| Pro[P] |
11 |
3.0% |
| Ser[S] |
26 |
7.2% |
| Thr[T] |
20 |
5.5% |
| Trp[W] |
4 |
1.1% |
| Tyr[Y] |
12 |
3.3% |
| Val[V] |
20 |
5.5% |
| Total Length |
362 |
|
| Instability index (II) |
39.04 |
| This classifies the protein as stable |
|
| Carbon |
1864 |
| Hydrogen |
2952 |
| Nitrogen |
508 |
| Oxygen |
559 |
| Sulfur |
7 |
| Total number of atoms |
5890 |
| Formula = C1864H2952N508O559S7 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
40130 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
39880 M-1 cm-1 |
| assuming all Cys residues are reduced |
39880 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
40130 M-1 cm-1 |
|
|
| Codon Bias |
-0.061 |
| Codon Adaptation Index |
0.108 |
| Frequency of Optimal Codons |
0.383 |
| Hydropathicity of Protein |
-0.510 |
| Aromaticity Score |
0.094 |
|
